DURAClone IM Phenotyping Basic Principle of the Panel Design
Markers
*Also detectable in 780/60 bandpass used in the CytoFlex and several of the CytoFLEX S series instruments.
Specificity | Purpose | Clone | Fluorochrome | Laser | Filter |
CD45 | Leukocyte gating | J33 | Krome Orange | 405nm | 525/40 |
CD3 | T cell lineage | UCHT1 | APC-Alexa Flour 750 | 638 nm | 763/43* |
CD4 | T Helper cell lineage | 13B8.2 | APC | 638 nm | 660/10 |
CD8 | Cytotoxic T cell lineage | B9.11 | Alexa Fluor 700 | 638 nm | 712/25 |
CD19 | B cell lineage | J3-119 | ECD (PE-Texas Red) | 488 nm | 610/20 |
CD14 | Monocyte lineage | RM052 | PE-Cy7 | 488 nm | 763/43* |
CD16 | NK cell lineage | 3G8 | FITC | 488 nm | 525/40 |
CD56 | NK cell lineage | N901 | PE | 488 nm | 585/42 |
*Also detectable in 780/60 bandpass used in the CytoFlex and several of the CytoFLEX S series instruments.
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