스윙형 로터
스윙형 원심분리기 로터(Swinging-bucket rotor)는 샘플의 긴 이동거리(pathlength), 비교적 긴 실행 시간 및 탁월한 해상도를 특징으로 합니다.
스윙형 로터는 원심력이 작용하게 되면 버킷의 각도(튜브의 기울기)가 변하게 됩니다. 버킷은 미사용 시 수직 형태로, 실행 중에는 수평 형태을 유지합니다. 스윙형 버킷의 수평형 형태는 샘플이 튜브의 벽에 부딪히는 고정각형 로터와 달리 샘플에 손상없이 밀도 기울기를 통해 분리할 수 있습니다.
많은 양의 샘플을 낮은 속도로 분리하는 경우에 적합하며, 샘플을 밀도에 따라 분리할 때 특히 유용합니다. 적용 가능한 작업에는 조직 배양 처리, 성장 과정에서 전체 세포 채취, 혈액 튜브 처리 및 밀도구배분리(크기 또는 질량 기반) 매체처럼 처리량이 많은 프로토콜이 포함됩니다.
FAQ on Swinging-Bucket Rotors
Why use a swinging-bucket rotor for density gradient centrifugation?
Density gradient centrifugation requires samples to separate into distinct layers based on density. Because swinging-bucket rotors position tubes horizontally during operation, they help create uniform gradients and well-defined sample bands, making them a preferred choice for many gradient-based separation workflows.
What applications are swinging-bucket rotors commonly used for?
Swinging-bucket rotors are frequently used for density gradient centrifugation, blood processing, bioprocessing workflows, and when highly purified samples are required . They are often selected when maintaining sample integrity and achieving high separation quality are important.
How does a swinging-bucket rotor work?
In a swinging-bucket rotor, sample tubes begin in a vertical position and gradually move to a horizontal position during centrifugation. This orientation allows particles to travel evenly through the sample, making swinging-bucket rotors especially suitable for density gradient separations and applications that require distinct sample layers.
What is the difference between a swinging-bucket rotor and a fixed-angle rotor?
A swinging-bucket rotor holds samples horizontally during centrifugation, making it well suited for density gradient separations and applications that require well-defined sample layers. They can also be used for pelleting and result in the pellet forming at the very bottom of the tube. A fixed-angle rotor keeps tubes at a constant angle and is typically preferred for rapid pelleting applications. The best choice depends on the separation objective and sample type.
What is a swinging-bucket rotor?
A swinging-bucket rotor is a centrifuge rotor that allows sample buckets to pivot during centrifugation. As rotational speed increases, the buckets swing outward into a horizontal position, creating a consistent linear sedimentation path that supports efficient sample separation.
