Characterizing Viral Vectors: Analyzing rAAV Vector Homogeneity

The solution to measuring rAAV vector homogeneity, purity (and more): analyze viral particles in solution.

Recombinant adeno-associated viral vectors (rAAV) could hold great promise for new, life-saving gene therapies.

But while classic techniques such as electron microscopy and Southern Blots can characterize rAAV heterogeneity and aggregation, they simply don’t provide sufficient resolution for quantifying homogeneity and viral particle load. When it comes to producing clinical-grade rAAV vector preparations, one obstacle has always been achieving high-resolution measurements.

Viral Vector CharacterizationAs gene therapy researchers know, with so much at stake in early product development, failing fast is fundamental. But up until now, the usual technologies have made that impossible when it comes to producing clinical-grade rAAV vectors.

The main obstacle? Lack of a high-resolution, quantitative technique for monitoring therapeutic quality with regard to:

  • Homogeneity
  • Purity
  • Product consistency
  • Viral particle load

The new solution: in-solution analysis with analytical ultracentrifugation (AUC).

As scientists such as those at Genethon have discovered, by enabling matrix-free analysis of rAAV vector preparations—independent of serotype and transgene—AUC helps them:

  • Determine viral assembly state or homogeneity (empty, full, oligomer)
  • Empirically quantify aggregation
  • Determine subparticle contamination
  • Quantify mass to accurately measure genetic payload

View this webinar to learn how Genethon’s Christine LE BEC and her team have used AUC to successfully characterize scAAV and ssAAV vectors—including homogeneity and viral particle load.

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