Although size selection protocols developed by other organizations do exist, we cannot guarantee performance or support this application. We suggest using the SPRIselect reagent, as it is developed specifically for size selection purposes. Visit this page to learn more.

The main difference between the SPRIselect and AMPure XP bead-based reagents is the intended use. The SPRIselect reagents are designed and validated for accurate and consistent DNA size selection from lot to lot, while the AMPure XP reagents are primarily validated for PCR purification and NGS cleanup. Learn more on this page.

The reagent will bind DNA and RNA; however, the RNAClean XP reagent is manufactured under RNase-free conditions and is QC tested to be RNase free whereas the AMPure XP reagent is not.

The AMPure XP reagent is manufactured and tested for the storage temperature indicated on the bottle and we can guarantee performance only at that temperature.

No. Any beads carried over to the final destination plate are inert in downstream enzymatic reactions.

Yes, but the recovery will decrease with a decrease in elution volume. In addition, if the beads cannot reach the magnet during the separation it may be necessary to leave a portion of the eluate behind if bead carryover must be avoided. Be sure to resuspend the beads fully in the elution buffer.

The binding capacity of the beads is so high that they cannot be exceeded with any sample type. It is possible to bind at least 7 μg of nucleic acid to 1 μL AMPure XP reagent, however the sample will become so viscous that it is difficult to pipette the sample.

The recovery depends on the fragment size, sample volume, sample concentration, elution volume and some other factors. Under ideal conditions recovery can be up to 90%, but under sub-optimal it can be as low as 60%.

Our beads rely upon a multifactorial equilibrium chemistry for binding, washing, and elution. Binding buffer components facilitate nucleic acid immobilization at functional-group-rich binding sites on the beads and the wash steps preserve this delicate equilibrium while solubilizing contaminants to allow for their removal. Elution disrupts this balance and re-solubilizes nucleic acids to allow for their separation from the beads.
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A dry step can be incorporated after the removal of the last ethanol wash and before the addition of the elution buffer. For purification of very large fragments, such as gDNA, a dry step is not recommended because recovery would be decreased. Fragments up to 40 kb have been known to tolerate a dry step.

Find Beckman Coulter Life Sciences AMPure XP technical documents here.